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目的研究大蒜油(garlic oil,GO)对正己烷(n-hexane)染毒大鼠CYP2E1、CYP2B1和ADH的影响及其机制。方法雄性Wistar大鼠50只,随机分为正常对照组、大蒜油对照组、正己烷模型组、正己烷+大蒜油低、高剂量组(n=10);模型组、大蒜油低、高剂量组大鼠分别给予2000 mg/kg·bw正己烷灌胃,大蒜油低、高剂量组大鼠于正己烷灌胃前1 h分别给予40和80 mg/kg·bw大蒜油灌胃,大蒜油对照组给予80 mg/kg·bw大蒜油灌胃,每周6次,持续10周。每2周测步态评分,监测大鼠周围神经损伤情况。10周末,检测肝组织中CYP2E1、CYP2B1和乙醇脱氢酶(ADH)表达及活性,以及血清中2,5-己二酮(2,5-HD)含量变化。结果与模型组相比,大蒜油低高剂量组步态评分分别降低了38.2%和41.2%(P<0.05),血清2,5-HD含量分别降低47.7%和78.7%(P<0.01);肝脏CYP2E1含量及活性分别降低了32.9%、39.1%和27.4%、44.5%(P<0.01);肝脏CYP2B1含量及活性高剂量组升高了57.2%和52.6%(P<0.01);肝脏ADH含量变化不明显,但活性显著降低了25.3%和86.0%(P<0.01)。结论大蒜油能保护正己烷所致的大鼠周围神经损伤,机制可能与肝脏CYP2E1、CYP2B1及ADH表达或活性改变,正己烷转化为2,5-HD减少有关。
Objective To study the effect and mechanism of garlic oil (GO) on CYP2E1, CYP2B1 and ADH in n-hexane-exposed rats. Methods Fifty male Wistar rats were randomly divided into normal control group, garlic oil control group, n-hexane model group, hexane + garlic oil low and high dose group (n = 10), model group, garlic oil low and high dose The rats were given 2000 mg / kg · bw n-hexane gavage, garlic oil low, high-dose group 1 h before n-hexane were given 40 and 80 mg / kg · bw garlic oil gavage, garlic oil The control group was given 80 mg / kg · bw garlic oil gavage 6 times a week for 10 weeks. The gait score was measured every 2 weeks to monitor the peripheral nerve injury in rats. At the end of the 10th week, the expression and activity of CYP2E1, CYP2B1 and ADH in the liver tissue and the content of 2,5-hexanedione (2,5-HD) in serum were detected. Results Compared with the model group, the gait score of garlic oil low dose group decreased by 38.2% and 41.2% (P <0.05) and the serum 2,5 - HD content decreased by 47.7% and 78.7% (P <0.01), respectively. The content and activity of CYP2E1 in liver decreased by 32.9%, 39.1% and 27.4%, 44.5% (P <0.01), respectively. The levels of liver CYP2B1 and high activity group increased by 57.2% and 52.6% The changes were not obvious, but the activity decreased significantly by 25.3% and 86.0% (P <0.01). Conclusion Garlic oil can protect the peripheral nerve injury induced by n-hexane, which may be related to the change of liver CYP2E1, CYP2B1 and ADH expression or activity, and the conversion of n-hexane to 2,5-HD.