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桑科植物Morus alba的药材,包括桑叶、桑枝、桑白皮(根皮)均含有多种多羟基生物碱,多羟基生物碱具有良好的降血糖、降血脂、抗病毒、提高免疫力、抗衰老等药理作用,但多羟基生物碱与在大多数植物中常见的糖类物质结构也相似,且具有强极性及碱性,所以其分离纯化将是复杂艰难的工作,分离步骤繁琐,通常需要多次柱色谱。查阅文献后,还未见到有关分离多羟基生物碱固定相材料方面的综述。因此,作者就桑类多羟基生物碱的分离,包括:反相、正相、离子交换、亲水色谱方面进行了综述,且针对每种分离方法都分析了其优点与不足,并提出以硅胶键合离子液体为新型吸附材料的柱色谱的方法,这种新型材料具有与亲水色谱中两性离子固定相类似的结构特点,不同点是该固定相中阴阳离子之间是离子键连接,同时其显示出特殊的分离特性-“两性”的特点,以期为桑类药材中多羟基生物碱的分离提供帮助以及为新型桑类药物的开发提供有价值的参考,成功分离多羟基生物碱也将为不同类型糖尿病的病人更针对性地用药提供条件。最后对新型硅胶键合离子液体固定相用于分离多羟基生物碱的发展和应用前景进行展望,其突出的优势为在色谱分析中,可根据离子液体的可调控性,来设计性的修饰固定相。
Morus alba mulberry plants, including mulberry, mulberry, Morus alba (root bark) contain a variety of polyhydroxy alkaloids, polyhydroxy alkaloids with good hypoglycemic, hypolipidemic, anti-virus, improve immunity , Anti-aging and other pharmacological effects, but polyhydroxy alkaloids and common in most plants in the sugar structure is also similar, and has a strong polarity and alkaline, so the separation and purification will be complex and difficult work, the separation steps cumbersome , Usually requires multiple column chromatography. After reviewing the literature, no review has been made on the separation of polyhydroxy alkaloid stationary phase materials. Therefore, the authors reviewed the separation of mulberry polyhydroxy alkaloids, including: reverse phase, normal phase, ion exchange, hydrophilic chromatography, and analyzed their advantages and disadvantages for each separation method, The bonded ionic liquid is a new column chromatography method for adsorbents. The novel material has the similar structural characteristics as the amphoteric immobilization in hydrophilic chromatography except that the cation and cation in the stationary phase are ionically bonded, meanwhile, It shows the special characteristics of separation - “sex”, with a view to help the separation of polyhydroxy alkaloids in mulberry herbs and to provide valuable references for the development of new mulberry drugs. The successful separation of polyhydroxy alkaloids It will also provide conditions for more targeted drug use among patients with different types of diabetes. Finally, the prospects for the development and application of the novel silica-bonded ionic liquid stationary phase for the separation of polyhydroxy alkaloids are prospected. The outstanding advantages of the novel silica gel-bonded ionic liquid stationary phases are that they can be designed and modified according to the regulatability of ionic liquids in chromatographic analysis phase.