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炎症性肠病(inflammatory bowel disease,IBD)病因虽未明确,但目前认为,肠道细菌和肠黏膜免疫功能紊乱与IBD的发病密切相关。将40只SD大鼠分为健康对照组、模型组、粪便微生物系移植组(fecal microbiota transplantation,FMT)和柳氮磺胺吡啶组,后3组用2,4,6-三硝基苯磺酸(2,4,6-trinitrobenzene sulfonic acid,TNBS)灌肠造模,造模2 d后分别用粪便悬液和柳氮磺胺吡啶治疗1 w。末次给药后禁食1 d,对大鼠粪便进行菌群成分分析,股动脉取血,对K+、Na+、血清白蛋白(ALB)、白细胞计数(WBC)、中性粒细胞百分率(N%)、C-反应蛋白(CRP)、IL-1β、IL-10、IL-12和IL-17水平进行检测,取结肠行病理学检查。结果发现,通过TNBS灌肠成功建立大鼠实验性结肠炎模型。与模型组比较,FMT组的K+和ALB明显升高(P<0.05),WBC、N%和CRP明显降低(P<0.05),IL-1β和IL-17明显降低(P<0.05),IL-10和IL-10/IL-12含量升高(P<0.05)。FMT能显著改善TNBS引起的肠道菌群变化,促进双歧杆菌的增殖而抑制脆弱拟杆菌和大肠杆菌的生长。上述结果证明,FMT可有效治疗炎症性肠病,其机制与其影响血清炎症因子水平和改善肠道菌群有关。
Although the etiology of inflammatory bowel disease (IBD) is not yet clear, it is currently believed that the immune function of intestinal bacteria and intestinal mucosa is closely related to the pathogenesis of IBD. Forty SD rats were divided into healthy control group, model group, fecal microbiota transplantation group (FMT) and sulfasalazine group, the latter three groups were treated with 2,4,6-trinitrobenzene sulfonic acid (2,4,6-trinitrobenzene sulfonic acid, TNBS) enema model, 2 days after modeling with stool suspension and sulfasalazine treatment 1 w. After the last administration, the rats were fasted for 1 day, and the feces of the rats were subjected to the analysis of the composition of the feces and the blood of the femoral artery. The K +, Na +, albumin (ALB), white blood cell count (WBC), neutrophil percentage (N% ), C-reactive protein (CRP), IL-1β, IL-10, IL-12 and IL-17 levels were measured. Colon pathological examination was performed. As a result, it was found that rat experimental colitis model was successfully established by TNBS enema. Compared with model group, K + and ALB in FMT group were significantly increased (P <0.05), WBC, N% and CRP were significantly decreased (P <0.05), IL-1β and IL- -10 and IL-10 / IL-12 levels increased (P <0.05). FMT can significantly improve the intestinal flora caused by TNBS, promote the proliferation of Bifidobacterium and inhibit the growth of Bacteroides fragilis and Escherichia coli. The above results demonstrate that FMT is effective in treating inflammatory bowel disease and its mechanism is related to its effect on serum levels of inflammatory cytokines and improvement of intestinal microflora.