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leaves was isolated and purified with DEAE cellulose 52,Sephacryl 300,heat treatment and preparative isotachophoresis. The molecular weight of AFP was 50 ku by SDS-PAGE measurement. The average thermal hysteresis activity (THA) of AFP was 0.35℃ at 5 mg/mL using a dif-ferential scanning calorimeter (DSC). This AFP showed heated stability. From circular dichroism (CD) of AFP spec-tral data from 195-240 nm,a well-defined secondary struc-ture of 11% a-helix,34% antiparallel b-sheet and 55% ran-dom coil for the plant AFP was deduced.