论文部分内容阅读
目的探讨β连环素(βcat)、上皮钙黏附素(Ecad)和黏着斑激酶(FAK)在大肠癌发生、发展中的作用。方法采用免疫组织化学方法检测了12例正常大肠黏膜,28例大肠腺瘤及49例大肠癌组织的βcat、Ecad和FAK表达情况。结果大肠癌βcat异位表达率61.2%,显著高于大肠腺瘤(35.7%P<0.05)。大肠癌βcat、Ecad膜表达缺失率分别为67.3%,57.1%,显著高于正常大肠黏膜和大肠腺瘤(P<0.01,P<0.01)。大肠癌FAK阳性率65.3%,高于大肠腺瘤和正常大肠黏膜(P<0.01)。βcat异位表达、βcat和Ecad膜表达缺失、FAK表达与大肠癌的浸润程度、淋巴结转移和Dukes分期等因素有关。大肠癌中,FAK表达与βcat、Ecad膜表达缺失及βcat异位表达呈正相关。结论βcat异位表达可能参与了大肠癌的发生、发展。βcat和Ecad膜表达缺失、βcat异位表达、FAK表达可能与大肠癌的侵袭和转移有关,此过程中,FAK可能抑制了癌细胞之间的黏附。
Objective To investigate the role of β-catenin, Ecad and FAK in carcinogenesis and progression of colorectal cancer. Methods The expressions of βcat, Ecad and FAK in 12 cases of normal colorectal mucosa, 28 cases of colorectal adenoma and 49 cases of colorectal carcinoma were detected by immunohistochemistry. Results The ectopic expression of βcat in colorectal cancer was 61.2%, significantly higher than that in colorectal adenoma (35.7%, P <0.05). The loss rates of βcat and Ecad in colorectal cancer were 67.3% and 57.1%, respectively, which were significantly higher than those in normal colorectal mucosa and colorectal adenoma (P <0.01, P <0.01). The positive rate of FAK in colorectal cancer was 65.3%, higher than that in colorectal adenoma and normal colorectal mucosa (P <0.01). βcat ectopic expression, lack of expression of βcat and Ecad membrane, FAK expression and the degree of invasion of colorectal cancer, lymph node metastasis and Dukes stage and other factors. Colorectal cancer, FAK expression and βcat, Ecad membrane expression loss and βcat ectopic expression was positively correlated. Conclusion The ectopic expression of βcat may be involved in the occurrence and development of colorectal cancer. The loss of βcat and Ecad membrane expression, βcat ectopic expression, FAK expression may be related to the invasion and metastasis of colorectal cancer, FAK may inhibit the adhesion between cancer cells in this process.