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目的 :研究补肾活血液对模型大鼠骨质疏松的影响及其机制 ,为临床中医药治疗骨质疏松提供实验依据。方法 :SD雌性大鼠 5 0只 ,随机分为 5组 :正常组 ,模型加生理盐水组 ,雌激素组 ,中药高、低剂量组。采用切除卵巢诱导的骨质疏松模型 ,用灌胃法给服补肾活血液 ,并以尼尔雌醇及生理盐水作为对照。各组动物均在 12周后处死 ,测定尿总羟脯氨酸 (U Hyp)、尿肌苷 (U Cr)、骨钙素 (BGP)、股骨抗弯强度(FBS) ,处死前测定全身骨密度 (GBMD)。结果 :中药组的GBMD及FBS明显高于模型组 (P <0 0 5 ) ,但低于正常组 (P <0 0 5 ) ;BGP明显高于模型组 (P <0 0 5 ,P <0 0 1) ,在高剂量时接近正常组 ;U Hyp/Cr明显低于模型组 (P <0 0 5 ) ,但仍然高于正常组 (P <0 0 5 )。结论 :补肾活血液对模型鼠骨质疏松有明显的抑制作用 ,主要影响骨形成环节。
Objective: To study the effect and mechanism of Bushen Huoxue blood on osteoporosis in model rats, and to provide experimental evidence for the clinical treatment of osteoporosis by traditional Chinese medicine. Methods: 50 female SD rats were randomly divided into 5 groups: normal group, model plus normal saline group, estrogen group, high and low dose Chinese medicine group. The ovariectomy-induced osteoporosis model was used to administer kidney-activating blood by gavage, and nilestriol and saline were used as controls. Animals in each group were sacrificed after 12 weeks. Urinary total hydroxyproline (U Hyp), urinary inosine (U Cr), osteocalcin (BGP), and femur bending strength (FBS) were measured. Body bone was measured before sacrifice. Density (GBMD). Results: The GBMD and FBS of the Chinese medicine group were significantly higher than those of the model group (P <0 05), but lower than the normal group (P <0 05); BGP was significantly higher than the model group (P <0 05, P <0 0 1), close to the normal group at high dose; U Hyp/Cr was significantly lower than the model group (P <0 05), but still higher than the normal group (P <0 05). Conclusion : Bushenhuoxue has obvious inhibitory effect on osteoporosis in model rats, which mainly affects the bone formation process.