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目的:探讨survivin,PTEN与结直肠癌细胞增殖和凋亡的关系及其在结直肠癌发生、发展中的作用.方法:应用流式细胞术测定正常肠黏膜、腺瘤、结直肠癌中survivin,PTEN表达及结直肠癌细胞凋亡;用免疫组织化学法测定结直肠癌细胞Ki67表达.结果:Survivin在正常肠黏膜中无表达,在腺瘤中表达率为18.4±3.4,结直肠癌中表达率为56.7±5.2,两者相比差异有显著性(P<0.05).PTEN阳性率由正常肠黏膜(87.6±3.1)、结直肠腺瘤(66.4±3.2)、结直肠癌组织(36.3±3.4)呈逐渐降低趋势(P<0.05).结直肠癌中survivin,PTEN的表达均与肿瘤分化程度相关(P<0.05),而与Dukes分期和淋巴结转移不相关(P>0.05).结直肠癌的凋亡指数,Survivin高表达者明显低于低表达者(P<0.05);PTEN高表达者明显高于低表达者(P<0.05).Ki67标记的增殖指数,Survivin高表达者明显高于低表达者(P<0.05);PTEN高表达者明显低于低表达者(P<0.05).结直肠癌组织中survivin与PTEN的表达呈负相关(rs=-0.846,P<0.05).结论:结直肠癌中survivin,PTEN通过调控细胞增殖和凋亡在结直肠癌的发生、发展中扮演着重要角色.
Objective: To investigate the relationship between survivin, PTEN and the proliferation and apoptosis of colorectal cancer cells and its role in the occurrence and development of colorectal cancer.Methods: The expressions of survivin, PTEN and colorectal cancer were detected by flow cytometry , PTEN expression and colorectal cancer cell apoptosis.Immunohistochemistry was used to detect the expression of Ki67 in colorectal cancer cells.Results: Survivin was not expressed in normal intestinal mucosa, the expression rate was 18.4 ± 3.4 in adenomas, The positive rate of PTEN was significantly higher than that in normal mucosa (87.6 ± 3.1), colorectal adenoma (66.4 ± 3.2), and colorectal cancer (36.3 ± 5.2) ± 3.4) (P <0.05) .The expression of survivin and PTEN in colorectal cancer were correlated with tumor differentiation (P <0.05), but not with Dukes stage and lymph node metastasis (P> 0.05) The apoptosis index of rectal cancer, Survivin high expression was significantly lower than those with low expression (P <0.05), PTEN high expression was significantly higher than low expression (P <0.05) .Ki67 labeling proliferation index Survivin high expression was significantly (P <0.05), while the high expression of PTEN was significantly lower than that of low expression (P <0.05) There was a negative correlation between the expression of urvivin and PTEN (rs = -0.846, P <0.05) .Conclusion: Survivin and PTEN plays an important role in the development and progression of colorectal cancer through regulating cell proliferation and apoptosis.