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γ-干扰素是参与结核病发病的一个细胞因子,在结核病的发病机制中起着重要作用。在γ-干扰素第1个内含子 CA重复序列的5′末端有个 T/A 单核苷酸多态性(+874 T/A 多态性),与微卫星等位基因2的出现和缺乏相关。我们利用聚合酶链反应-序列特异性引物(PCR-SSP)技术对肺结核病患者及对照人群γ-干扰素+874 T/A 多态性进行检测,以探讨该基因多态性与肺结核易感性的关系。对象与方法病例组60例肺结核患者的诊断标准参照文献[1-2],根据病史、症状及体征、X 线胸片、痰结核分枝杆菌涂片确诊,年龄18～84岁(中位数为54.1岁),其中男42例,女18例;初治45例,复治15例;痰结核分枝杆菌阳性24
Interferon gamma is a cytokine involved in the pathogenesis of tuberculosis and plays an important role in the pathogenesis of tuberculosis. There was a T / A SNP (+874 T / A polymorphism) at the 5 ’end of the first intron CA repeats of IFN-γ, which coincided with the appearance of microsatellite allele 2 And lack of relevance. We used polymerase chain reaction-sequence-specific primer (PCR-SSP) to detect IFN-γ +874 T / A polymorphism in patients with pulmonary tuberculosis and control subjects to explore the polymorphism of the gene and susceptibility to pulmonary tuberculosis Relationship. Patients and Methods 60 cases of pulmonary tuberculosis patients with diagnostic criteria reference [1-2], according to history, symptoms and signs, X-ray, sputum Mycobacterium tuberculosis smear diagnosis, aged 18 to 84 years (median 54.1 years old), including 42 males and 18 females; initial treatment in 45 cases, retreatment in 15 cases; positive sputum Mycobacterium tuberculosis 24