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目的用生物反应器制备乙脑纯化灭活疫苗。方法用搅拌式生物反应器培养Vero细胞,接种并培养乙型脑炎病毒,将收获的病毒液进行灭活和纯化。结果细胞密度都增至2.4×106ml-1以上,病毒滴度都>7.25lgLD50/ml。结论用生物反应器制备乙脑灭活纯化疫苗的工艺合理,可以生产出高滴度的乙型脑炎病毒液。
Objective To prepare JE purified inactivated vaccine by using bioreactor. Methods Vero cells were cultured in agitated bioreactor, inoculated and cultured with encephalitis virus, and the harvested virus solution was inactivated and purified. The results of cell density increased to 2.4 × 106ml-1 above, the virus titer> 7.25lgLD50 / ml. Conclusion The bioreactor preparation of JE inactivated purified vaccine process is reasonable, can produce high titers of encephalitis virus solution.