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目的:研究复方中药对非酒精性脂肪肝肝细胞色素P450ⅡE1表达的影响。方法:用高脂饲料诱导大鼠脂肪肝模型,同时给于复方中药干预,观察肝组织病理形态的变化和肝细胞色素P450ⅡE1的表达,测定肝MDA和SOD、GSH、Vit E以及TG含量的变化,并与对照组比较。结果:中药组的肝组织脂肪变性基本恢复正常.免疫组化证明复方中药能显著抑制脂肪肝肝细胞色素P450ⅡE1的表达,同时肝内MDA和SOD、GSH、Vit E及TG的含量回复到接近正常,结论:该复方中药能显著抑制脂肪肝肝细胞色素P450ⅡE1表达.因而具有防止脂肪肝的作用
Objective: To study the effect of compound Chinese herbs on the expression of hepatic cytochrome P450IIE1 in nonalcoholic fatty liver. METHODS: Rat model of fatty liver was induced by high-fat diet. At the same time, compound Chinese herbs were intervened to observe the pathological changes of hepatic tissue and the expression of hepatic cytochrome P450IIE1. The changes of hepatic MDA, SOD, GSH, Vit E and TG were determined. And compared with the control group. Results: The hepatic tissue steatosis of the Chinese medicine group basically returned to normal. Immunohistochemistry proved that the compound Chinese herbs can significantly inhibit the expression of hepatic cytochrome P450IIE1 in fatty liver, and the content of MDA and SOD, GSH, Vit E and TG in the liver returned to near normal. Conclusion: The compound Chinese herbs can significantly inhibit the expression of hepatic cytochrome P450IIE1 in fatty liver, and thus have the effect of preventing fatty liver.