T淋巴细胞常被用作机体细胞免疫功能的一个重要指标,常用的检测方法是羊红细胞玫瑰花结试验,但小鼠的T淋巴细胞的结花率很低。Mueller等(1975)首先应用酯酶细胞化学染色技术鉴别T淋巴细胞,并很快得到了许多实验室研究资料的支持。T淋巴细胞的胞浆里含有α—醋酸酯酶(ANAE),能水解α—醋酸萘酯生成萘酚,后者与六偶氮付品红偶联生成点状或块状的棕红色沉淀物,它是T细胞所特有的标志。B细胞无此种反应。 T lymphocytes are often used as an important indicator of cellular immune function. The commonly used test is the Rattus test, but the rate of T lymphocytes in mice is low. Mueller et al. (1975) first used esterase cytochemical staining to identify T lymphocytes and was quickly supported by many laboratory studies. T lymphocyte cytoplasm contains α -acetic acid esterase (ANAE), which can hydrolyze α-naphthyl acetate to produce naphthol, the latter coupled with hexaazaphthalene to produce dotted or massive brown-red precipitate , Which is a hallmark of T cells. B cells have no such reaction.