论文部分内容阅读
目的：观察α＿１－ＡＲ的减敏过程及其亚型差异。方法：给清醒大鼠持续输注苯肾上腺素（ＰＥ）及离体主动脉和肾动脉用去甲肾上腺素（ＮＥ），持续温育后，测定血压或进行血管收缩功能实验。结果：持续输注ＰＥ后，由ＰＥ介导的升压作用较对照组明显降低，升高相同血压所需的ＰＥ剂量显著增加；离体血管经不同时间和不同浓度的ＮＥ预温育后，α＿１－ＡＲ介导的血管收缩－效应曲线（ＣＲＣ）显著右移，但含α＿（１Ｄ）－ＡＲ的主动脉的右移程度均显著大于含。α＿（１Ａ）－ＡＲ的肾动脉。结论：α＿１－ＡＲ在激动剂持续作用下可产生减敏，且α＿（１Ｄ）－ＡＲ远较α＿（１Ａ）－ＡＲ容易发生。
Objective: To observe the desensitization process and the subtypes of α_1-AR. Methods: Phenylephrine (PE) and norepinephrine (NE) were transfused into awake rats continuously. After continuous incubation, blood pressure and vasoconstriction were measured. RESULTS: After continuous infusion of PE, the PE-mediated vasopressor effect was significantly lower than that of the control group, and the PE dose required for increasing the same blood pressure was significantly increased. Exposed blood vessels were preincubated with NE at different times and different concentrations, α_1-AR-mediated vasoconstriction-response curve (CRC) shifted significantly to the right, but the right aorta containing α_ (1D) -AR shifted significantly more than the right. α_ (1A) -AR renal artery. CONCLUSION: α_1-AR can produce desensitization under the action of agonist, and α_ (1D) -AR is far more likely than α_ (1A) -AR.