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目的探讨不同粒径、不同浓度的纳米钴对鼠成纤维细胞的遗传毒性效应。方法分别用含有1μg/ml、10μg/ml、100μg/ml浓度的30nm、50nm和100nm的钴悬液处理培养的鼠成纤维细Balb/c3T3,24h后,采用单细胞凝胶电泳实验方法检测DNA损伤,评价遗传毒性效应。结果与对照组比较,30nm、50nm和100nm的钴颗粒,当浓度大于1μg/ml时均可对细胞产生遗传毒性效应,差异及显著(P<0.01),且随着浓度的增加毒性作用增强。结论纳米钴对小鼠成纤维细胞具有遗传毒性作用,且遗传毒性与纳米钴浓度呈剂量依赖效应。
Objective To investigate the genotoxic effects of cobalt nanoparticles of different particle sizes and different concentrations on murine fibroblasts. Methods The cultured rat fibroblast fine Balb / c3T3 cells were treated with 30nm, 50nm and 100nm cobalt suspensions containing 1μg / ml, 10μg / ml and 100μg / ml respectively for 24 hours. The DNA was detected by single cell gel electrophoresis Injury, evaluation of genotoxic effects. Results Compared with the control group, cobalt nanoparticles at 30nm, 50nm and 100nm showed genotoxic effects on cells at a concentration higher than 1μg / ml (P <0.01), and the toxicity was enhanced with the increase of concentration. Conclusion Nanocrystalline cobalt has a genotoxic effect on mouse fibroblasts, and the genotoxicity has a dose-dependent effect on the concentration of nanocrystalline cobalt.