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目的观察骨髓间充质干细胞(BMSCs)移植对急性期脑梗死大鼠的干预作用。方法按随机化原则将50只脑梗死大鼠模型随机分为BMSCs移植组、上清液移植组各22只及对照组6只。BMSCs组在脑梗死大鼠模型成功后3 h经静脉注入BMSCs 1 ml,上清液组注入上清液1 ml,对照组注入磷酸盐缓冲液(PBS)1 ml。术后28 d检测PBMSc在脑内的存活转化情况。分别于0、7、14及28 d观察记录各组大鼠的神经损伤严重程度评分(NSS)。结果 28 d后,BMSCs脑梗死灶边缘可见少量BrdU阳性细胞表达神经元特异性烯醇化酶(NSE),而上清液组及对照组病灶边缘则无NSE;细胞移植后,各组神经功能均有不同程度的恢复;从第7天起,BMSCs移植组NSS评分明显低于上清液组及对照组(P<0.05),而后2组之间差异无统计学意义(P>0.05)。结论骨髓间充质干细胞移植入急性脑梗死大鼠体内后,可存活、移行至梗死灶周围,并分化为神经元细胞,而且能够减轻脑梗死大鼠的神经损伤程度。
Objective To observe the effects of bone marrow mesenchymal stem cells (BMSCs) transplantation on acute cerebral infarction in rats. Methods According to the principle of randomization, 50 rats with cerebral infarction were randomly divided into BMSCs transplantation group, 22 supernatant transplantation group and 6 control group. In the BMSCs group, 1 ml of BMSCs was infused intravenously 3 h after the successful cerebral infarction model. The supernatant group was injected with 1 ml of supernatant and the control group with 1 ml of PBS. The survival of PBMSc in the brain was detected on the 28th postoperative day. The severity of neurological injury (NSS) was recorded on the 0, 7, 14 and 28 d groups respectively. RESULTS: After 28 days, a small amount of BrdU-positive neurons were expressed on the edge of cerebral infarction in BMSCs, while neuronal specific enolase (NSE) was not found in the supernatant group and the control group. After transplantation, (P <0.05). The NSS score of BMSCs transplantation group was significantly lower than that of the supernatant group and the control group from the 7th day (P <0.05), but there was no significant difference between the 2 groups (P> 0.05). CONCLUSION: Bone marrow mesenchymal stem cells can survive and migrate to the periphery of infarcted area and differentiate into neurons after transplanted into the body of rats with acute cerebral infarction, and can reduce the degree of nerve damage in rats after cerebral infarction.