【摘 要】
：
Using a degenerated primer and a T-primer, a MADS-box gene, M79,was amplified by RT-PCR from rice fluorescence at meiosis stage and then cloned. Sequence analys
【机 构】
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National Laboratory of Protein Engineering and Plant Genetic Engineering,National Laboratory of Gene
【基金项目】
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The authors would like to thank Xihui Liang and Dr.Honghong Zheng fortheir help in preparation of this manuscript
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Using a degenerated primer and a T-primer, a MADS-box gene, M79,was amplified by RT-PCR from rice fluorescence at meiosis stage and then cloned. Sequence analysis shows that M79 shares 98.2% homology with OsMADS7 at DNA level while only 92% at the amino acid level. The transcript of M79 possesses five different polyadenylation sites. Only a single copy of M79 gene has been found in rice genome, which is located on chromosome 8. M79 is expressed specifically in flower organs, from pre-meiosis stage through pollen maturation. Ectopic expression of M79 in T0 and T1 transgenic rice results in early-flowering, implying that M79 is involved in controlling the flowering time. In the same time, M79 may be involved in controlling the branching process to make more flower buds.
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