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目的:探讨瘦素、雌激素(E2)及受体(ob-R,ER)在子宫内膜异位症(EM)发病中的作用。方法:采用放射免疫法和免疫组织化学法检测30例EM患者和30例非EM妇女(对照组)血清中瘦素、E2的水平和组织中瘦素、ob-R和ER的蛋白表达情况。结果:EM患者血清瘦素和E2水平与对照组均无明显差异(3.49±1.19μg/Lvs3.50±1.31μg/L,P1>0.05;67.79±21.95pg/mlvs60.51±20.25pg/ml,P2>0.05)。不同期别的子宫内膜中各蛋白表达:在增生期亚组,瘦素在EM组在位和异位内膜组织中表达均明显低于对照组(P1<0.05,P2<0.05),ER在EM组在位内膜组织中表达明显高于对照组(P<0.05),而ob-R在EM组和对照组中的表达无明显差异;在分泌期亚组,EM组在位内膜组织中瘦素和ob-R表达明显低于对照组(P1<0.05,P2<0.05),ER表达高于对照组(P<0.05),而EM组异位内膜组织中各蛋白的表达与对照组无明显差异。结论:EM患者在位内膜组织中瘦素、ob-R和ER表达的变化,可能为EM的发生、发展中起到了一定的作用。
Objective: To investigate the role of leptin, estrogen (E2) and receptor (ob-R, ER) in the pathogenesis of endometriosis (EM). Methods: The levels of leptin, E2 and the expression of leptin, ob-R and ER in 30 EM patients and 30 non-EM women (control group) were detected by radioimmunoassay and immunohistochemistry. Results: The levels of serum leptin and E2 in EM patients were not significantly different from those in the control group (3.49 ± 1.19μg / L vs3.50 ± 1.31μg / L, P> 0.05; 67.79 ± 21.95pg / ml vs60.51 ± 20.25pg / ml, P2> 0.05). The expression of leptin in eutopic and ectopic endometrium in EM group was significantly lower than that in control group (P <0.05, P <0.05), ER The expression of ob-R in the eutopic endometrium of EM group was significantly higher than that of the control group (P <0.05), while the expression of ob-R in the EM group and the control group had no significant difference. In the secretory phase subgroup, The expression of leptin and ob-R was significantly lower than that of the control group (P <0.05, P <0.05), and the expression of ER was higher than that of the control group (P <0.05) No significant difference in the control group. Conclusion: The changes of leptin, ob-R and ER expression in eutopic endometrium of EM patients may play a role in the occurrence and development of EM.