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[目的]探讨细胞周期蛋白D1(Cyclin D1)与细胞周期蛋白D3(Cyclin D3)在非小细胞肺癌(NSCLC)中的表达及临床意义。[方法]免疫组织化学法检测Cyclin D1、Cyclin D3蛋白在271例NSCLC及相应正常组织中的表达情况,分析其相关性及其与临床特征的关系。[结果 ]Cyclin D1和Cyclin D3定位于细胞质和细胞核中,癌组织与正常组织中阳性表达率差异显著(P均为0.000),同一患者癌组织中Cyclin D3表达高于Cyclin D1。Cyclin D1、Cyclin D3蛋白表达在不同性别、年龄、吸烟史、肿瘤大小及位置、病理分型组中均无统计学差异(P均>0.05)。在不同病理分级、临床TNM分期及淋巴结是否转移组中,Cyclin D1表达均无统计学差异(P>0.05),而Cyclin D3表达差异均显著(P<0.05)。在总NSCLC、腺癌、鳞癌中,Cyclin D1和Cyclin D3表达的相关性检验显示两者均具有正相关性,r分别为0.332、0.377、0.308(P均为0.000)。Cyclin D1低表达患者和高表达患者的中位生存时间接近,无统计学差异(P=0.264);Cyclin D3低表达患者的中位生存时间较高表达者明显延长,差异有统计学意义(P=0.001)。[结论 ]Cyclin D1和Cyclin D3表达可能与NSCLC发生相关,但在NSCLC发展与转移等恶性生物学行为中,Cyclin D3起重要作用,并可作为评估NSCLC恶性生物学行为及预后的参考指标。
[Objective] To investigate the expression and clinical significance of Cyclin D1 and Cyclin D3 in non-small cell lung cancer (NSCLC). [Method] The expression of Cyclin D1 and Cyclin D3 proteins in 271 cases of NSCLC and its corresponding normal tissues were detected by immunohistochemistry, and their correlations and their relationship with clinical features were analyzed. [Results] Cyclin D1 and Cyclin D3 were localized in the cytoplasm and nucleus. The positive expression rates of Cyclin D1 and Cyclin D3 in cancer tissues were significantly different from those in normal tissues (all P <0.0001). The expression of Cyclin D3 in the same patient was higher than that in Cyclin D1. The expressions of Cyclin D1 and Cyclin D3 were not significantly different in different gender, age, smoking history, tumor size and location, pathological type (P> 0.05). There was no significant difference in the expression of Cyclin D1 (P> 0.05), but there was significant difference in the expression of Cyclin D3 (P <0.05) in different pathological grades, clinical TNM stage and lymph node metastasis. In NSCLC, adenocarcinoma and squamous cell carcinoma, the correlation between the expression of Cyclin D1 and Cyclin D3 showed a positive correlation (r = 0.332,0.377,0.308, P = 0.000). The median survival time of patients with low expression of Cyclin D1 was significantly higher than that of patients with high expression (P = 0.264). The median survival time of patients with low expression of Cyclin D1 was significantly longer (P = 0.001). [Conclusion] The expression of Cyclin D1 and Cyclin D3 may be related to the occurrence of NSCLC. However, Cyclin D3 plays an important role in the malignant biological behaviors such as the development and metastasis of NSCLC, and can be used as a reference index to evaluate the malignant biological behavior and prognosis of NSCLC.