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目的探讨Bnip-3、Bcl-2在实验性糖尿病心肌病中的表达。方法16只雄性成年Wistar大鼠随机分为糖尿病组(10只)与对照组(6只),糖尿病组一次性腹腔注射链脲佐菌素(STZ)溶液诱导建立糖尿病模型,对照组腹腔注射枸橼酸缓冲液。12周后处死大鼠,取血检测血脂、心肌肌钙蛋白-I,切取心室肌组织HE染色观察,并随后行免疫组化染色,观察Bnip-3、Bcl-2的表达情况。结果血生化指标及心肌形态学改变均符合糖尿病心肌病表现。免疫组化染色显示Bnip-3主要阳性表达部位为细胞核和细胞质,糖尿病组Bnip-3阳性表达率明显高于对照组〔(57.70±12.77)%vs(36.68±5.86)%,P<0.01〕;Bcl-2主要阳性表达部位为细胞膜和/或细胞质,糖尿病组Bcl-2阳性表达率明显低于对照组〔(22.34±12.70)%vs(74.03±10.45)%,P<0.01〕。结论Bnip-3在糖尿病心肌细胞中表达增强,具有促进心肌细胞凋亡的作用;Bcl-2呈现弱表达,其抑制心肌细胞凋亡作用减弱,二者均加速了心肌细胞的凋亡。
Objective To investigate the expression of Bnip-3 and Bcl-2 in experimental diabetic cardiomyopathy. Methods Sixteen male adult Wistar rats were randomly divided into diabetic group (n = 10) and control group (n = 6). Diabetic rats were given a single intraperitoneal injection of streptozotocin (STZ) solution to induce diabetes mellitus. Citrate buffer. After 12 weeks, the rats were sacrificed, blood was taken for determination of serum lipids and cardiac troponin-I, and the ventricular myocytes were cut out for HE staining. Immunohistochemical staining was performed to observe the expression of Bnip-3 and Bcl-2. Results Blood biochemical parameters and myocardial morphological changes were in line with the performance of diabetic cardiomyopathy. The positive expression of Bnip-3 in the diabetic group was significantly higher than that in the control group [(57.70 ± 12.77)% vs (36.68 ± 5.86)%, P <0.01]. Immunohistochemical staining showed that the main positive expression sites of Bnip-3 were nucleus and cytoplasm. The main positive expression sites of Bcl-2 were cell membrane and / or cytoplasm. The positive rate of Bcl-2 in diabetic group was significantly lower than that in control group (22.34 ± 12.70% vs 74.03 ± 10.45%, P <0.01). Conclusions The expression of Bnip-3 is enhanced in diabetic cardiomyocytes, which may promote the apoptosis of cardiomyocytes. Bcl-2 is weakly expressed, which inhibits the apoptosis of cardiomyocytes, both of which accelerate the apoptosis of cardiomyocytes.