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OBJECTIVE To validate an ELISA method for the determination of adiponectin in cynomolgus monkeys.The ELISA kit was commercially available, but originally designed for the measurement of adiponectin in humans.METHODS The assay was a sandwich ELISA configured with a capture monoclonal antibody immobilized on the wells of a microtiter plate and a detection monoclonal antibody labeled with horseradish peroxidase.Tetramethylbenzidine was used as the substrate of horseradish peroxidise and the color absorbance was measured at two wavelengths (450 nm and 540 nm).Sample volume of 50 I was required for each determination.