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目的:探讨法舒地尔(FAS)对高糖培养的人腹膜间皮细胞(HMrSV)RhoA/Rock1、活性氧(ROS)、GSH-Px及转化生长因子β1(TGF-β1)表达的影响。方法:将人腹膜间皮细胞株HMrSV分为对照组(葡萄糖浓度为0)、高糖组(葡萄糖浓度分别为1.5%、2.5%和4.25%)和FSA干预组(2.5%葡萄糖+FAS 12.5、25和50μmol/L)。检测方法:Q-PCR法和Western印迹法检测RhoA和Rock1的表达;化学荧光法检测ROS的含量;ELISA法检测GSHPx和TGF-β1的含量。结果:与对照组相比,高糖组可呈浓度依赖方式上调RhoA、Rock1、ROS及TGF-β1的表达,下调GSH-Px的表达;与2.5%高塘组相比,FAS干预可明显下调RhoA、Rock1、ROS及TGF-β1的表达,上调GSH-Px的表达。结论:FAS可能通过抑制Rho激酶活性抑制高糖培养的人腹膜间皮细胞发生氧化应激反应。
Objective: To investigate the effect of fasudil on the expression of RhoA / Rock1, reactive oxygen species (ROS), glutathione peroxidase (GSH-Px) and transforming growth factor β1 (TGF-β1) in cultured human peritoneal mesothelial cells (HMrSV) Methods: Human peritoneal mesothelial cell line HMrSV was divided into control group (glucose concentration 0), high glucose group (glucose concentration 1.5%, 2.5% and 4.25% respectively) and FSA intervention group (2.5% glucose + FAS 12.5, 25 and 50 μmol / L). Detection methods: Qho-PCR and Western blotting were used to detect the expression of RhoA and Rock1; ROS was detected by chemiluminescence method; GSHPx and TGF-β1 were detected by ELISA. Results: Compared with the control group, the high glucose group could up-regulate the expression of RhoA, Rock1, ROS and TGF-β1 and down-regulate the expression of GSH-Px in a concentration-dependent manner. RhoA, Rock1, ROS and TGF-β1, upregulated the expression of GSH-Px. Conclusion: FAS may inhibit oxidative stress in human peritoneal mesothelial cells cultured in high glucose by inhibiting Rho kinase activity.