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目的比较低氧浓度(5%O2)与常规氧浓度(20%O2)胚胎培养在体外受精(in vitro fertilization,IVF)或卵胞浆内单精子注射(Intracytoplasmic sperm injection,ICSI)中对胚胎早期发育及妊娠结局的影响。方法本中心195例IVF/ICSI周期接受常规促排卵,取卵后随机分为低氧浓度组与常规氧浓度组分别培养,观察其受精情况、胚胎发育情况、冷冻情况及妊娠结局。结果低氧组共纳入周期101例,常氧组94例。两组正常受精率、多精受精率、D1卵裂率、D2D3优胚率比较均无统计学差异(P>0.05);两组新鲜移植率、胚胎冷冻率、解冻移植率比较均无统计学差异(P>0.05);两组新鲜移植周期妊娠率,低氧组高于常氧组(χ2=3.963,P<0.05),两组解冻移植周期妊娠率相比无统计学差异。结论低氧浓度胚胎培养可一定程度提高IVF/ICSI妊娠结局,但仍需大样本的随机对照研究进一步证实。
Objective To compare the effects of low oxygen (5% O2) and conventional oxygen (20% O2) embryos on early embryo development in in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) Development and outcome of pregnancy. Methods The 195 IVF / ICSI cycles of our center received routine ovulation induction. After ovulation, they were randomly divided into hypoxia group and conventional oxygen concentration group. The fertilization, embryo development, freezing and pregnancy outcome were observed. Results A total of 101 cycles were included in hypoxia group and 94 in normoxia group. There was no significant difference in normal fertilization rate, multiple fertilization rate, D1 cleavage rate and D2D3 excellent embryo rate (P> 0.05). There was no significant difference in fresh implantation rate, embryo freezing rate and thawing rate between the two groups (P> 0.05). The pregnancy rate of fresh transplantation in both groups was higher than that in normoxia group (χ2 = 3.963, P <0.05). There was no significant difference in the pregnancy rate between the two groups in thawed transplantation. Conclusions Embryonic hypoxia can improve pregnancy outcome of IVF / ICSI to a certain extent, but still need large sample randomized controlled study further confirmed.