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DNA分子标记技术是检测遗传差异的一种新技术 ,特别是RAPD技术 ,应用更为广泛。DNA质量是保证RAPD分析成功的关键。本文用改进的SDS法对沙拐枣属植物种子的总DNA进行了提取。琼脂糖凝胶电泳结果表明 :得到的DNA片段大小在 2 0kb以上 ;通过PCR扩增实验 ,证明用此方法提取的DNA ,可直接用于随机扩增的DNA多态性 (RAPD)遗传标记
DNA molecular marker technology is a new technology to detect genetic differences, especially RAPD technology, which is more widely used. DNA quality is the key to successful RAPD analysis. In this paper, the improved SDS method was used to extract the total DNA of the seeds of Calligonum. The results of agarose gel electrophoresis showed that the size of the obtained DNA fragment was above 20kb. The PCR amplification proved that the DNA extracted by this method can be directly used for random amplified polymorphic DNA (RAPD) genetic markers