神经节的培养一般采用两种方法,即培养皿法和双盖片法。双盖片法是将培养的神经节贴附在胶原盖片上,而后将贴附有神经节的盖片又贴在云母片或玻片上,最后神经节上滴加培养液,放在凹玻片内,云母片与凹玻片周围用蜡封好,放入37℃温箱内培养。双盖片培养法是比较古典的培养方法,优点是:方法容易掌握;培养小室是密封的,所以培养过程不易污染。但它的缺点是:加入培养小室内的培养液少,培养2～3天就需更换新的培养液,在更换培养液时将云母片从凹玻上起下来,换新凹玻片和云母片,最后加入新培养液封好培养。结果操作繁琐,有可能造成污染。为了克服双盖片培养法的缺点,我们对 Ganglion culture generally use two methods, namely culture dish method and double-cover method. Double-cover method is to train the ganglion attached to the collagen cover, and then attached to the ganglion flap and attached to the mica or slide, the last ganglion drip culture medium, on the concave slide Inside, mica and concave glass slide around the wax seal, placed in 37 ℃ incubator. Double-cover culture method is more classical culture methods, the advantage is: the method is easy to grasp; culture chamber is sealed, so the culture process is not easy to pollution. But its disadvantage is: adding less culture medium culture chamber, cultured 2 to 3 days on the need to replace the new culture medium, the replacement of culture solution mica from the concave glass up, renewal of concave glass and mica Finally, add new culture medium and seal well. Results of the operation cumbersome, may cause pollution. In order to overcome the shortcomings of the double-cover culture method, we are right