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采用热水煮提法和乙醇沉淀法从穿山龙中提取出水溶性粗多糖,再经DEAE-Sepharose CL-6B和Sepha-dex G-100柱层析分离纯化得到2个均多糖组分DMA和DMB。利用超氧阴离子实验,羟基阴离子实验和邻苯三酚自氧化实验研究DMA和DMB的抗氧化活性,结果表明DMB有较强的抗氧化活性,应该被作为潜在的抗氧化剂开发利用。
The water-soluble crude polysaccharides were extracted from Chimonanthus pontoniae by hot boiling method and ethanol precipitation method, and then two polysaccharides, DMA and DMB, were isolated and purified by DEAE-Sepharose CL-6B and Sephadex G-100 column chromatography. The antioxidant activities of DMA and DMB were studied by superoxide anion, hydroxyl anion and pyrogallol autoxidation. The results showed that DMB has strong antioxidant activity and should be exploited as a potential antioxidant.