论文部分内容阅读
目的响应曲面法优化黄花败酱总皂苷(TSPS)的提取工艺及研究TSPS促进大鼠离体肠平滑肌收缩活性。方法以齐墩果酸为对照品,采用香草醛.冰醋酸显色,利用紫外分光光度计在550 nm处测定TSPS的量。以TSPS提取率作为响应值,在单因素试验的基础上采用响应曲面法考察提取溶剂、料液比、提取时间及其交互作用对TSPS提取效果的影响。并且测定了优化提取工艺过程中获取的不同TSPS提取物的促肠平滑肌收缩活性,结合生物活性全面评价TSPS提取工艺。结果TSPS的最佳提取工艺条件为提取溶剂75%乙醇,料液比1:12,提取时间为1.5 h,提取温度90℃、提取2次。在此工艺条件下TSPS的提取率达到10.96 mg/g,其对大鼠离体肠平滑肌的收缩作用强弱表现为结肠>回肠>空肠>十二指肠,质量浓度达0.32 mg/mL时促进离体肠收缩效果最佳。结论经优化后的TSPS提取工艺条件具有提取率高,提取工艺高效、稳定等特点,且TSPS具有显著的促进大鼠离体肠平滑肌收缩功能,其功效与提取率呈正相关。
Objective To optimize the extraction process of total saponin (TSPS) of Patrinia dahurica by response surface methodology and to study the TSPS-induced contractile activity of isolated smooth muscle in rats. Methods Oleanolic acid was used as reference substance, and vanillin and glacial acetic acid were used for colorimetric determination. The amount of TSPS was measured by UV spectrophotometer at 550 nm. TSPS extraction rate was used as a response value. Response surface methodology was used to investigate the effects of extraction solvent, solid-liquid ratio, extraction time and their interaction on the extraction of TSPS based on single factor experiments. In addition, the contractile activity of different intestinal TSPS extracts obtained during the optimized extraction process was determined, and the TSPS extraction process was comprehensively evaluated in combination with biological activity. Results The optimal extraction conditions of TSPS were extraction solvent 75% ethanol, solid - liquid ratio 1:12, extraction time 1.5 h, extraction temperature 90 ℃ and extraction twice. TSPS extraction rate of 10.96 mg / g under this condition was higher than that of the control group (P> 0.05). The contractile effect of TSPS on the isolated rat intestinal smooth muscle was in the form of colon> ileum> jejunum> duodenum. When the concentration of TSPS was 0.32 mg / Intestinal gut contraction best. CONCLUSION TSPS extraction process has the advantages of high extraction rate, high efficiency and stability of extraction process. TSPS can significantly promote contractile function of isolated rat intestinal smooth muscle, and its efficacy is positively correlated with extraction rate.