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采用体外细胞毒性试验的方法研究了全氟辛酸(PFOA)对人肺A549细胞的毒性作用。结果表明:当PFOA质量浓度≥50μg/mL时,细胞皱缩变圆,并随PFOA质量浓度增加,细胞间隙变大,细胞变圆的数量增多;PFOA对A549细胞的增殖存在抑制作用,抑制作用与PFOA质量浓度呈正相关;PFOA会导致细胞内活性氧(ROS)含量升高,PFOA染毒质量浓度与细胞内ROS含量呈正相关;PFOA可诱导细胞凋亡,细胞凋亡率随PFOA质量浓度升高呈缓慢上升趋势。PFOA对人肺癌细胞具有毒性作用,可诱导细胞凋亡,且PFOA可随大气颗粒物进行长距离迁移,其对人体呼吸系统的影响或发生致毒效应的风险应当引起重视。
The toxicity of perfluorooctanoic acid (PFOA) to human lung A549 cells was studied by in vitro cytotoxicity assay. The results showed that when the mass concentration of PFOA was ≥50μg / mL, the cell shrinkage became round, and with the increase of PFOA mass concentration, the cell gap became larger and the number of round cells increased; PFOA had inhibitory and inhibitory effects on the proliferation of A549 cells And PFOA concentration was positively correlated; PFOA could lead to the increase of intracellular reactive oxygen species (ROS), PFOA concentration and intracellular ROS content; PFOA can induce apoptosis, the rate of apoptosis with PFOA mass concentration High slowly upward trend. PFOA has toxic effects on human lung cancer cells and induces apoptosis. PFOA may migrate with atmospheric particles over long distances, and its impact on human respiratory system or the risk of toxic effects should be taken seriously.