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为了探索影响高原粳稻区白叶枯病菌变异的因子,以2个云南高原粳稻区白叶枯菌株2001-2、K-1为供试菌株,在毫糯扬、金南风、滇粳优5号和合系41等不同品种组合上连续接种或连续交叉接种后分离菌株,利用高原粳稻白叶枯病菌的鉴别品种和分子检测手段,对获得的菌株的致病型及基因变异进行分析。结果显示,原始菌株2001-2与其7个获得菌系(23个菌株)的致病型都不同,其中,除获得菌系6-2的3个菌株致病力增强外,其他6个获得菌系的20个菌株致病力均减弱;而原始菌株K-1的抗感反应模式与其获得菌系6-8的7个菌株完全相同。利用IS1112、hrpF引物进行菌株PCR扩增以及UPGMA聚类分析并将相应序列测序,结果显示,供试菌株IS1112、hrpF分子谱型的遗传分簇与菌株致病型之间无一一对应关系;在基因序列上,各菌株的IS1112和hrpF的两个序列片段表现出不同的碱基变化特征。两个原始菌株接种在不同抗性品种组合上获得的菌系的致病性变异具有特殊性和不定向性。
In order to explore the factors influencing the variation of bacterial blight in japonica rice in the plateau, two strains of Bacillus subtilis 2001-2 and K-1 from japonica rice in Yunnan Plateau were used as tested strains. No. 41 and other combinations of different combinations of continuous inoculation or continuous cross-inoculation after isolating strains, the use of isolates of Xanthomonas campestris isolates and molecular detection means to analyze the pathogenicity of the strains and genetic variation were analyzed. The results showed that the pathogenicity of the original strain 2001-2 was different from that of the seven strains (23 strains). Among them, the pathogenicity of the three strains except strain 6-2 was enhanced, while the other 6 strains The pathogenicity of the 20 strains was weakened. The anti-susceptibility pattern of the original strain K-1 was exactly the same as that of the strains obtained from strains 6-8. The IS1112 and hrpF primers were used for PCR amplification and UPGMA cluster analysis and sequencing of the corresponding sequences. The results showed that there was no one-to-one correspondence between genetic clustering and strain pathogenicity of IS1112 and hrpF strains. In the gene sequence, two sequences of IS1112 and hrpF of each strain showed different base change characteristics. Pathogenic variation of two original strains inoculated on combinations of different resistant varieties was special and non-directional.