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目的 :建立能诱导脑缺血耐受的缺血预处理SD大鼠模型。方法 :SD大鼠分别给予生理盐水右侧颈内动脉灌注 (SI)、双侧颈总动脉夹闭 (BCAO)和双侧颈总动脉夹闭并生理盐水右侧颈内动脉灌注 (BCAO +SI)预处理 ,每次持续 3min ,间隔 7min ,反复 3次 ,2 4h后作线栓大脑中动脉栓塞 (MCAO)。观察处理对MCAO缺血对侧肢体活动、脑组织含水量和梗死体积的影响。结果 :MCAO后 2 4h和 48hBCAO +SI预处理组神经损害体征和脑含水量较SI和BCAO两组轻 ;MCAO 72hBCAO +SI组脑梗死体积也低于其他两组。结论 :BCAO +SI预处理能诱导脑组织明显缺血耐受 ,是研究缺血耐受机制比较理想的缺血预处理模型
Objective: To establish a rat model of ischemic preconditioning induced by cerebral ischemia. Methods: SD rats were given right internal carotid artery perfusion (SI), bilateral common carotid artery occlusion (BCAO) and bilateral common carotid artery occlusion and normal saline right internal carotid artery perfusion (BCAO + SI ) Pretreatment, each time for 3min, interval 7min, repeated 3 times, 24 hours after thread occlusion of the middle cerebral artery occlusion (MCAO). To observe the effect of MCAO treatment on contralateral limb activity, brain tissue water content and infarct volume. Results: The signs of neurological damage and brain water content in MCAO 72 h BCAO + SI group were lower than those in SI and BCAO groups at 24 h and 48 h BCAO + SI pretreatment. CONCLUSION: Preconditioning with BCAO + SI can induce obvious ischemic tolerance in brain tissue and is an ideal ischemic preconditioning model for studying the mechanisms of ischemia tolerance