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在人工气候室进行的不同温度、不同相对湿度条件下花期牙签接种试验的结果表明 :温度和相对湿度显著地影响病斑的长度。在中、英、法三国对中国和欧洲的 14个油菜品系进行的抗性鉴定结果表明 :不同品系植株病斑的平均长度有差异 ,说明不同品系抗菌核病能力有差异。牙签接种法能够根据接种后一定时间病斑的平均长度区分品种的抗菌核病能力。且方法简单 ,快速 ,易操作。但由于接种过程中植株茎受到人为创伤 ,其外部防御系统遭到了破坏 ,使植株的抗侵染和抗扩展能力有所改变。建议在使用牙签穿刺法鉴定的同时 ,结合使用别的菌核病抗性鉴定方法。
The results of flowering toothpick inoculation experiments under different temperatures and different relative humidity in the artificial climate chamber showed that the temperature and the relative humidity significantly affect the length of the lesion. The results of resistance evaluation of 14 rapeseed lines in China and Europe in China, Britain and France showed that there were differences in the average length of the diseased plaques among different lines, indicating that there are differences in the abilities of different strains of BCB. Toothpick inoculation method according to a certain period of time after inoculation the average length of the lesion to distinguish between varieties of antibacterial nuclear capacity. And the method is simple, fast, easy to operate. However, due to human inoculation of plant stems during the inoculation process, the external defense system has been destroyed, so that plant resistance to infection and resistance to change has changed. Recommended toothpick puncture method used in the same time, combined with the use of other Sclerotinia resistance identification method.