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目的建立辣椒油和火锅底料中的10种工业染料的检测方法。方法采用凝胶渗透色谱仪作为前处理设备,采用超高效液相色谱仪作为检测设备,对高脂肪含量的辣椒油及火锅底料等辣椒制品中的10种红黄类工业染料进行检测,并对方法的有效性进行验证。结果以空白的辣椒油和火锅底料为基质,分别在高、中、低三个水平进行加标实验,回收率分别在56.0%~96.4%之间和70.0%~98.2%之间,RSD(n=6)分别为1.9%~8.2%之间和1.8%~7.9%之间,各工业染料的检出限(S/N=3)在0.009~0.051 mg/kg之间,定量限(S/N=10)在0.030~0.153 mg/kg之间。结论该方法操作简单,稳定性好,适于在实际检测工作中推广应用。
Objective To establish a method for the detection of 10 industrial dyes in chilli oil and pot bottom material. Methods Gel permeation chromatography (GPC) was used as pretreatment equipment. Ultra-high performance liquid chromatography (HPLC) was used as detection equipment to detect 10 kinds of red and yellow industrial dyes in chili products such as high fat content chili oil and pot bottom material. The validity of the method is verified. Results The blank spicery oil and pot base material were used as the matrix. The spiked experiments were carried out at high, medium and low levels respectively. The recoveries were between 56.0% and 96.4% and between 70.0% and 98.2%, respectively. RSD ( n = 6) were between 1.9% and 8.2% and between 1.8% and 7.9% respectively. The detection limits (S / N = 3) of all the industrial dyes ranged from 0.009 to 0.051 mg / / N = 10) is between 0.030 and 0.153 mg / kg. Conclusion The method is simple, stable and suitable for general application in practical testing.