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在建立了大鼠喉移植动物模型的基础上,本研究对环孢素 A(CsA)的肝肾毒性作用进行了形态学观察。突验共分3组,接受 CsA 治疗的两个实验组的大鼠,移植术当天和术后分别经腹腔注射 CsA15mg·kg~(-1)/d 和25mg·kg~(-1)/d,对照组未用 CsA 治疗。2周后将受体处死,取肝肾组织进行组织病理学检查。结果表明:CsA 肾毒性损害主要表现为肾近曲小管上皮细胞空泡变性,肾细小动脉玻璃样变性,管壁增厚,肾间质不同程度纤维化。而肝的毒性损害则以肝细胞脂肪变性为主,伴轻度的间质细胞增生,偶见肝细胞局灶性坏死,上述病理改变与 CsA 的应用剂量呈正相关。
On the basis of establishing animal model of rat laryngeal transplantation, the morphological changes of hepatotoxicity induced by cyclosporin A (CsA) were observed in this study. The experimental groups were divided into three groups. Rats in two experimental groups receiving CsA were injected intraperitoneally with 15 mg · kg -1 of CsA and 25 mg · kg -1 of d The control group was not treated with CsA. After 2 weeks, the recipient was sacrificed, and liver and kidney tissues were taken for histopathological examination. The results showed that nephrotoxicity of CsA was mainly manifested as vacuolar degeneration of renal proximal tubule epithelial cells, vitreous degeneration of renal arterioles, thickening of the wall and varying degrees of fibrosis of the renal interstitium. The liver toxicity damage is mainly based on the steatosis of liver cells, accompanied by mild interstitial cell hyperplasia, occasional hepatic cell necrosis, the above pathological changes and CsA dose was positively correlated.