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目的：观察静脉内滴注谷氨酰胺（Ｇｌｎ）对急性重症胰腺炎（ＡＳＰ）后肠源性细菌／内毒素易位的保护作用。方法：选健康长白种猪２１头，体重１６～２２ｋｇ，雌雄不限，随机分为四组。Ⅰ组：假手术对照组（ｎ＝５）；Ⅱ组：ＡＳＰ对照组（ｎ＝５）；Ⅲ组：ＡＳＰ＋甘氨酸（Ｇｌｙ）组（ｎ＝５）；Ⅳ组：ＡＳＰ＋Ｇｌｎ组（ｎ＝６）。在麻醉状态下，进腹向胰管内注入５％牛磺胆酸钠混合液１ｍｌ／ｋｇ（内含８０００～１００００ＢＡＥＥ单位胰蛋白酶／ｍｌ，ｐＨ７．６）诱导ＡＳＰ。以０．９％ＮａＣｌ磷酸盐缓冲液取代５％牛磺胆酸钠混合液即为假手术对照组。采集腔静脉血作内毒素测定（鲎试剂法）。ＡＳＰ后７２ｈ，采集门、腔静脉血作细菌定量培养和细菌鉴定。然后静脉推注１０％氯化钾２０ｍｌ处死后，立即进胸腹取大小肠系膜淋巴结、肺组织、肺门淋巴结、胰腺组织，称重后研磨成组织匀浆，并作细菌定量培养和细菌鉴定。结果：静脉内输注Ｇｌｎ后可使血浆中Ｇｌｎ浓度较Ⅰ组、Ⅱ组明显升高（Ｐ均＜０．０１）。静脉内Ｇｌｎ支持可明显降低血浆内毒素水平，且使血液和组织器官中易位的细菌数量均出现非常显著的下降。结论：静脉内Ｇｌｎ支持可显著地升高血浆Ｇｌｎ水平，可显著地减轻ＡＳＰ后肠?
Objective: To observe the protective effect of intravenous glutamine (Gln) on enterogenic bacterial / endotoxin translocation after acute severe pancreatitis (ASP). Methods: 21 healthy long white pigs weighing 16-22 kg were randomly divided into four groups. (N = 5); GroupⅡ: ASP control group (n = 5); GroupⅢ: ASP + Gly group (n = 5); GroupⅣ: ASP + Gln group . Under anesthesia, into the abdominal cavity into the pancreatic duct 5% sodium taurocholate mixture 1ml / kg (containing 8000 ~ 10000BAEE units trypsin / ml, pH7.6) induced ASP. Substitution of 5% sodium taurocholate in 0.9% NaCl phosphate buffer was sham-operated control. Collect vena cava blood for endotoxin determination (鲎 reagent method). 72h after the ASP, collection of doors, vena cava for bacterial quantitative culture and bacterial identification. Then intravenous injection of 10% potassium chloride 20ml after sacrifice, immediately into the chest and abdomen to take the size of mesenteric lymph nodes, lung tissue, hilar lymph nodes, pancreas, weighed and then ground into tissue homogenates, and bacterial quantitative culture and bacterial identification. Results: Gln concentration in plasma after intravenous infusion of Gln was significantly higher than that of group Ⅰ and group Ⅱ (all P <0.01). Intravenous Gln support can significantly reduce plasma endotoxin levels and cause a very significant decrease in the number of translocating bacteria in blood and tissues and organs. Conclusion: Intravenous Gln support can significantly increase plasma Gln levels, can significantly reduce the hindpaw of the ASP?