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本实验用100～200g雄性Wistar大白鼠21只,其中8只用5％的牛胆酸钠用常规手 术方法经胰导管注射入胰腺组织造成急性胰腺炎为造型组,7只在手术前5天胃饲0.5ml/100g.B.W/天大黄水煎剂,于第5天向胰导管注5％牛胆酸钠,此为防治组、6只为对照组,胰导管注射生理盐水,手术后24小时,颈椎脱臼处死动物,观察胰腺外形并从胰导管附近取胰腺组织迅速用CO_2固定,在-15℃制成冰冻切片,按Glenner方法观察单胺氧化酶,按Nachles方法观察琥珀酸脱氢酶。在Carnoy′s液内固定,按Cook方法观察DNA,
In this experiment, 21 male Wistar rats (100-200 g) were used. Eight of them were injected with pancreatic ducts into the pancreatic tissue with 5% sodium bilechol injection. The acute pancreatitis was caused by acute pancreatitis. Gastric feeding 0.5ml/100g.BW/day rhubarb water decoction, on the 5th day to the pancreatic duct injection 5% sodium bilecholate, this is the prevention group, 6 as the control group, pancreatic duct injection of saline, after surgery 24 Hours, the animals were sacrificed by cervical dislocation. The appearance of the pancreas was observed. Pancreatic tissue was taken from near the pancreatic duct and quickly fixed with CO2. Frozen sections were made at -15°C. Monoamine oxidase was observed by the Glenner method and succinate dehydrogenase was observed by the Nachles method. After immobilization in Carnoy’s solution, observe DNA by Cook method.