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目的通过观察大黄蒽醌提取物抑制小鼠病变肾组织纤维化的作用,探讨大黄治疗肾脏病的作用机制。方法采用左侧输尿管结扎的方法建立单侧输尿管梗阻(UUO)雄性CD-1 小鼠动物模型。用形态学半定量方法评价组织学病变;酸水解-比色法测定肾组织胶原的含量; 蛋白印迹技术检测胶原α表达的水平。以α-平滑肌肌动蛋白(α-SMA)作为上皮细胞转分化的观察指标。结果大黄提取物(50 mg／kg体重)能够显著地减少肾间质的病变,降低肾组织中胶原的聚积,与对照组相比两者差异均有统计学意义。大黄提取物(25、50 mg／kg体重)能够降低病变肾组织中胶原α的表达水平及减少梗阻肾组织中α-SMA的表达,抑制上皮细胞转分化。结论大黄蒽醌化合物能够改善肾脏的纤维化,其作用可能与降低肾组织内胶原的沉积以及抑制上皮细胞转分化有关。
Objective To investigate the mechanism of rhubarb treating kidney disease by observing the effect of rhubarb extract on inhibiting the pathological changes of renal tissue in mice. Methods A unilateral ureteral obstruction (UUO) male CD-1 mouse animal model was established by left ureteral ligation. Histological lesions were evaluated by morphological semi-quantitative methods; collagen content in renal tissues was measured by acid hydrolysis-colorimetry; and collagen alpha expression level was measured by Western blotting. Α-smooth muscle actin (α-SMA) was used as an index of transdifferentiation of epithelial cells. Results Rhubarb extract (50 mg/kg body weight) significantly reduced renal interstitial lesions and decreased collagen accumulation in renal tissue. There was a statistically significant difference between the two groups compared with the control group. Rhubarb extract (25, 50 mg/kg body weight) can reduce the expression level of collagen α in the diseased kidney tissue and reduce the expression of α-SMA in the obstructed kidney tissue, and inhibit epithelial cell transdifferentiation. Conclusion Rhubarb compound can improve renal fibrosis, and its effect may be related to the decrease of collagen deposition in renal tissue and inhibition of epithelial cell transdifferentiation.