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过去,已证明异种病毒可以加速人类免疫缺陷病毒-1(HIV-1)的感染过程。本实验用单纯疱疹病毒(HSV)感染ACH-2细胞(HIV-1持续感染的细胞)的实验模型,测定HSV是否能激活HIV的基因表达。所用的HSV包括野株(KOS)和缺失早期编码蛋白的突变株△ICP0、AICP4、AICP27。ACH-2细胞分别用各株HSV-1感染后,测定上清液中HIV p24抗原、病毒颗粒逆转录酶(RT)活性以及细胞中HIV特异RNA的表达。KOS感染72h后,HIVp24抗原含量及RT活性分别为
In the past, xenogenic viruses have been shown to accelerate the infection of human immunodeficiency virus-1 (HIV-1). In this experiment, an experimental model of ACH-2 cells (HIV-1 persistently infected cells) was infected with herpes simplex virus (HSV) to determine whether HSV can activate HIV gene expression. The HSV used included wild-type (KOS) and mutant strains ΔCCO0, AICP4, AICP27 that lacked the early-encoded protein. ACH-2 cells were infected with each strain of HSV-1, the determination of HIV p24 antigen in the supernatant, viral particle reverse transcriptase (RT) activity and HIV-specific RNA expression in cells. After 72h KOS infection, the HIVp24 antigen content and RT activity were respectively