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二点委夜蛾是近年来在我国黄淮海夏玉米区暴发为害的一种玉米新害虫.为了系统揭示二点委夜蛾种群在山东省的扩散途径与发生规律,本研究利用线粒体细胞色素氧化酶亚基I(mtCOI)基因对山东省12个地市的二点委夜蛾种群以及威海1个二点委夜蛾形态近似种种群进行遗传结构分析.结果表明:130条二点委夜蛾mtCOI基因(608 bp)共有24个单倍型,7条二点委夜蛾形态近似种的mtCOI基因共有2个单倍型.单倍型网络图与系统发育树显示,威海地区二点委夜蛾形态近似种与其他种群分为2大组,它们之间存在着显著遗传分化,遗传距离为0.044~0.054.AMOVA分析表明,山东省二点委夜蛾种群遗传变异主要来自组间,该地区二点委夜蛾并没有经历过种群扩张.该研究为此害虫的预测预报及防治提供了科学依据.
In order to systematically reveal the spreading pathways and occurrence regular of the armyworm population in Shandong Province, this study utilized mitochondrial cytochrome oxidase The enzyme subunit I (mtCOI) gene was used to analyze the genetic structure of a population of two members of the armyworm population in 12 cities in Shandong Province and one species of the litura in Weihai. The results showed that: There were 24 haplotypes in the mtCOI gene (608 bp) and 2 haplotypes in the mtCOI gene in seven similar morphological species of S. exigua. The haplotype network map and phylogenetic tree showed that the two-point night The moth morphological and other populations were divided into two groups, with significant genetic differentiation between them, and the genetic distance was 0.044 ~ 0.054.AMOVA analysis showed that the genetic variation of the population of the second instar larvae of Shandong was mainly from the inter-group, The second stage of the night-stage moth did not experience population expansion, which provided a scientific basis for the prediction and prevention of this pest.