金黄色葡萄球菌和A群链球菌可产生多种外毒素,这些外毒素具有超抗原（SAg）性能。细菌SAg对人体免疫系统有明显影响,严重时常造成危及生命的中毒性休克综合征。鉴于产SAg菌株耐药现象日趋广泛,研制有效的细菌SAg菌苗已提到议事日程。作者采用限制性位点专一诱变技术对细菌SAg的共有受体结合基元进行诱变。将突变基因插入表达质粒,转入大肠杆菌,扩增后冻融、溶菌酶处理得包涵颗粒,再裂解得突变型SAg重组蛋白。用DR1纯合性人B淋巴 Staphylococcus aureus and group A Streptococcus can produce a variety of exotoxins, these exotoxins with superantigen (SAg) performance. Bacteria SAg on the human immune system have a significant impact, often causing serious life-threatening toxic shock syndrome. In view of the increasingly wide range of drug-resistant SAg strains, the development of effective bacterial strains of SAg has been mentioned on the agenda. The authors used restriction site-specific mutagenesis to mutate the consensus receptor binding motif of bacterial SAg. The mutant gene was inserted into the expression plasmid, transformed into Escherichia coli, amplified and freeze-thawed, and lysozyme-treated to obtain the inclusion granule, and then the mutant SAg recombinant protein was cleaved. DR1 homozygous human B lymph