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目的：对肝动脉注射３２Ｐ－玻璃微球（３２Ｐ－ＧＭＳ）的肝细胞损伤进行电镜形态计量，为肝癌的放射介入治疗提供形态学依据。方法：７只家猪经肝动脉注射直径为４６～７６μｍ的ＧＭＳ，其中４只注射放射性３２Ｐ－ＧＭＳ，３只注射３１Ｐ－ＧＭＳ（对照）。术后２、４、１６周分批取不同部位的肝组织进行常规电镜制样和电镜形态计量分析。结果：３２Ｐ－ＧＭＳ术后２周和４周时，核异常率及线粒体变异率均明显高于３１Ｐ－ＧＭＳ术后２周、４周及热球术后１６周（Ｐ＜０．００１～０．０１）。热球术后２周和４周时，线粒体体密度明显低于术后１６周（Ｐ＜０．０１～０．０２），但与冷球术后２周和４周无明显差异。线粒体的其它形态参数，各组间均无明显差异。结论：吸收剂量低于２００Ｇｙ的３２Ｐ－ＧＭＳ，术后２周和４周时，肝细胞核及线粒体的损伤明显，随后逐渐恢复，至术后１６周时恢复正常。说明肝细胞的损伤是短时而可逆的。
OBJECTIVE: To observe the morphological changes of liver cells damaged by hepatic artery injection of 32P-GMS microspheres (32P-GMS), and provide morphological evidence for the interventional therapy of liver cancer. Methods: Seven domestic pigs were injected with GMS with a diameter of 46 ~ 76μm via the hepatic artery. Four of them were injected with radioactive 32P-GMS and three injected with 31P-GMS (control). At 2, 4, and 16 weeks after operation, liver tissues from different parts were routinely taken electron microscopy and electron microscopy. Results: The rates of nuclear abnormalities and mitochondrial variations were significantly higher at 32 weeks and 2 weeks after 32P-GMS treatment than those at 31 weeks after 2P, 4 weeks and 16 weeks after PTCA (P <0.001-0 .01). The mitochondrial body densities were significantly lower at 2 weeks and 4 weeks after hot ball operation than those at 16 weeks postoperatively (P <0.01-0.02), but there was no significant difference between 2 and 4 weeks after cold ball operation. Other morphological parameters of mitochondria, no significant differences between the groups. CONCLUSION: The 32P-GMS with absorbed dose lower than 200Gy showed obvious damage to the nucleus and mitochondria of liver cells at 2 weeks and 4 weeks after operation, then recovered gradually and returned to normal after 16 weeks. It shows that the damage of liver cells is short-term and reversible.