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目的：探讨不同分布区的蛇床Ｃｎｉｄｉｕｍ ｍｏｎｎｉｅｒｉ 的ＩＴＳ序列变异与其地理分布和化学成分的相关性。方法：设计２ 对引物，Ｐｆ＋ Ｐｂ 及Ｐ５－８ＳＩＴＳ１＋ Ｐ５－８ＳＩＴＳ２ ，ＰＣＲ扩增产物纯化后用银染法或ＡＢＩ３１０ 测序。结果：得到核糖体ＤＮＡ中的ＩＴＳ及５－８ＳｒＤＮＡ 完全序列，１８Ｓ和２６ＳｒＤＮＡ 部分序列，共约７００ ｂｐ 。５ 个地点样品的ＩＴＳ１及ＩＴＳ２ 的序列大小分别为２１０～２１７ ｂｐ 和２１９ ～２２４ ｂｐ。ＩＴＳ１ 碱基序列的遗传距离０－００ ～１－９３％ ，ＩＴＳ２ 碱基序列的遗传距离０－４６ ～２－３４％ ，ＩＴＳ１ 较为保守。以ＮＪ法根据ＩＴＳ２ 序列数据重建系统发生树。哈尔滨样品聚为一组，衡水与德州样品和郑州与高淳样品各自聚为一组。结论：ＩＴＳ２ 序列的变异与中国产蛇床的纬度分布相关，而其与蛇床化学型的关系尚需作进一步研究。
Objective: To investigate the relationship between ITS sequence variation and its geographical distribution and chemical composition of Cnidium monnieri in different distribution areas. Methods: Two pairs of primers, Pf+ Pb and P5-8SITS1+ P5-8SITS2, were designed and purified by PCR and sequenced with silver staining or ABI310. RESULTS: ITS and 5-8S rDNA complete sequences, 18S and 26S rDNA partial sequences in ribosomal DNA were obtained, totaling approximately 700 bp. The sequence sizes of ITS-1 and ITS-2 in the five sites were 210-217 bp and 219-224 bp, respectively. The genetic distance of the ITS-1 base sequence is 0-00 to 1-93%, and the genetic distance of the ITS-2 base sequence is 0-46 to 2-34%. ITS-1 is more conservative. The phylogenetic tree was reconstructed from the ITS-2 sequence data using the NJ method. Samples from Harbin gathered in one group. Hengshui and Texas samples and Zhengzhou and Sorghum samples were grouped together. Conclusion: The variation of ITS2 sequence is related to the latitude distribution of Cnidium monnieri in China, and its relationship with the chemical composition of Cnidium monnieri needs further research.