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本文研究了实验性高胆固醇血症大鼠肝及小肠中低密度脂蛋白（ＬＤＬ）受体ｍＲＮＡ和载脂蛋白（ａｐｏ）Ａ－１ｍＲＮＡ的水平变化以及苯甲酸雌二醇（ＥＢ）对二者的影响。发现高脂（ＨＣ）组肝及小肠ＬＤＬ受体ｍＲＮＡ水平分别低于正常组５０％和６０％（Ｐ＜０．０５），小肠ａｐｏＡ－１ｍＲＮＡ水平低于正常组５８％（Ｐ＜０．０５），此时血清总胆固醇（ＴＣ）及ＬＤＬ胆固醇（ＬＤＬ－ｃ）均明显高于正常组（Ｐ＜０．０１），血清ａｐｏＡ－１低于正常组（Ｐ＜０．０５）。ＨＣ＋ＥＢ组血清ＴＣ及ＬＤＬ－ｃ明显低于ＨＣ组，而肝ＬＤＬ受体ｍＲＮＡ水平则显著高于ＨＣ组，为ＨＣ组的３．５倍（Ｐ＜０．００２）。结果提示：（１）高胆固醇负荷时细胞可通过转录水平下行调节ＬＤＬ受体；（２）小肠可能在ａｐｏＡ－１的代谢中起重要作用；（３）ＥＢ可能通过诱导肝ＬＤＬ受体基因表达而降血脂。
In this paper, the changes of low density lipoprotein (LDL) receptor mRNA and apolipoprotein A-1 mRNA in the liver and small intestine of experimental hypercholesterolemic rats and the effect of estradiol benzoate (EB) Impact. The mRNA levels of LDL receptor in the liver and small intestine of high fat (HC) group were lower than those in normal group by 50% and 60%, respectively (P <0.05), and the apoA-1 mRNA in small intestine was lower than that in normal group by 58% ). Serum total cholesterol (TC) and LDL-cholesterol (LDL-c) were significantly higher than those in normal group (P <0.01), serum apoA-1 was lower than normal group (P <0.05). The levels of serum TC and LDL-c in HC + EB group were significantly lower than those in HC group, while the mRNA level of LDL receptor in liver was significantly higher than that in HC group (3.5 times, P <0.002). The results suggest that: (1) cells may down-regulate LDL receptor through transcription at high cholesterol load; (2) small intestine may play an important role in apoA-1 metabolism; (3) EB may induce hepatic LDL receptor gene expression And lipid-lowering.