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应用RAPD技术对红鳍笛鲷(♀)、千年笛鲷(♂)和红鳍笛鲷(♀)×千年笛鲷(♂)子一代3个群体的RAPD扩增带谱进行了分析,并对杂交子代F1杂种优势进行了预测。从100个随机引物中筛选出35个扩增带丰富的引物进行扩增,共得到190条清晰稳定的扩增带,其中有176个扩增位点具有多态性,多态性位点比率为92.6%。红鳍笛鲷、F1、千年笛鲷3个群体的遗传相似性指数分别为0.831 9,0.726 3,0.916 8;Nei多样性指数分别为0.174 5,0.264 0,0.118 4;Shannon信息指数分别为0.256 4,0.384 5,0.171 2。红鳍笛鲷(♀)-F1,F1-千年笛鲷(♂),红鳍笛鲷(♀)-千年笛鲷(♂)的遗传距离分别为0.397 2,0.317 0,0.854 1。从相似性指数、Nei多样性指数和Shannon信息指数均显示F1代有杂种优势,并找到了种间特异性标记。
RAPD technique was used to analyze the RAPD bands of three populations of red snapper (♀), millennium snapper (♂) and redfin snapper (♀) × millennium snapper (♂) Hybrid F1 progeny were predicted. A total of 190 clear and stable amplification bands were screened out from 100 random primers for amplification with abundant primers, of which 176 were polymorphic. The percentage of polymorphic loci 92.6%. The genetic similarity index of red fins snapper, F1 and millennium snapper were 0.831 9,0.726 3,0.916 8 respectively; Nei diversity index was 0.174 5,0.264 0,0.118 4; Shannon information index was 0.256 4,0.384 5,0.171 2. The genetic distances of red snapper (♀) -F1, F1-Millennium snapper (♂), red snapper (♀) -Midan snapper (♂) were 0.397, 2.0317 and 0.854 respectively. The similarity index, Nei diversity index and Shannon information index all showed that the F1 generation had the heterosis and found the interspecies specific marker.