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目的总结陈旧石蜡组织块的免疫组织化学染色及防脱片经验。方法选取包埋时间为4年的金黄地鼠胰腺和肝脏石蜡组织块各150个,按照本课题组改进的实验方法进行切片及免疫组织化学染色。结果镜下观察显示,切片染色阳性结构清晰,无非特异性背景着色,脱片率约为10%。结论陈旧石蜡组织块在自来水里浸泡3~4d后再切能够提高切片质量;在免疫组织化学中烤片、抗原修复、一抗均是影响脱片的关键环节。
Objective To summarize the experience of immunohistochemical staining and anti-shedding of obsolete paraffin blocks. Methods 150 pancreas and liver paraffin blocks of golden hamster with embedding time of 4 years were selected and sliced and immunohistochemically stained according to the improved experimental method of our group. Results Microscopic observation showed that the positive staining of the sections was clear and no non-specific background was colored. The rate of desquamation was about 10%. Conclusion Obsolete paraffin blocks can be dipped into tap water for 3 ~ 4 days before slicing to improve the slicing quality. In immunohistochemistry, baking chips, antigen retrieval and primary antibodies are the key factors affecting the delamination.