复方中药对代谢综合征SD大鼠的干预研究

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目的:观察益气活血化浊中药对代谢综合征(MS)模型大鼠的干预作用。方法:雄性SD大鼠60只,高脂高盐饲料喂养复制MS大鼠模型。随机分为正常组、模型组、文迪雅组、及中药(低、中、高剂量)组,中药组予中药、正常组和模型组予等体积0.9%氯化钠溶液灌胃;治疗4周后测血糖、胰岛素、血脂、瘦素、胰岛素敏感指数(ISI)、脂肪组织过氧化物增殖体激活受体(PPARγmRNA)表达。结果:①中药各组体质量、内脏脂肪重量及系数、瘦素水平均显著低于模型组(P<0.05,P<0.01),但文迪雅组降低无统计学意义;②中药中剂量和文迪雅组的餐后2h血糖、血脂均显著性低于模型组而ISI显著高于模型组(P<0.05);③中药中剂量组PPARγmRNA表达比值显著高于模型组(P<0.05),但低于文迪雅组(P<0.01)。结论:该复方中药促进MS大鼠脂肪组织PPARγmRNA表达作用虽弱于文迪雅,但兼有纠正糖脂代谢紊乱、减肥、改善胰岛素、瘦素抵抗等多重作用。 Objective: To observe the intervention of Yiqi Huoxue Huazhuo Chinese medicine on rats with metabolic syndrome (MS). Methods: Sixty male Sprague-Dawley rats were fed with high-fat and high-salt diet for the replication of MS rats. Were randomly divided into normal group, model group, Avandia group, and Chinese medicine (low, medium and high dose) groups. Traditional Chinese medicine group was given traditional Chinese medicine, normal group and model group were given 0.9% sodium chloride solution by gavage. Blood glucose, insulin, lipids, leptin, insulin sensitivity index (ISI) and adipose tissue peroxisome proliferator activated receptor (PPARγmRNA) expression were measured after a week. Results: (1) Body weight, visceral fat mass, coefficient and leptin level in each group were significantly lower than those in the model group (P <0.05, P <0.01), but there was no significant difference in the Wendya group In the Yaoya group, the blood glucose and blood lipid levels at 2h after meal were significantly lower than those in the model group and ISI was significantly higher than that in the model group (P <0.05); ③ The PPARγmRNA expression ratio in the medium dose group was significantly higher than that in the model group (P <0.05) Lower than Avandya group (P <0.01). Conclusion: The compound Chinese herbal medicine can promote PPARγmRNA expression in adipose tissue of MS rats even though it is weaker than that of Avandia, but it can both play a multiple role in correcting glucose and lipid metabolism, reducing weight, improving insulin and leptin resistance.
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