用三氯醋酸(TCA)及硫酸铵(AS)综合法从布氏菌菌液中制备了纯化的新型布氏菌素—布氏菌纯蛋白衍生物,简称“Br—PPD”。多次动物试验证明,Br—PPD具有良好的变态反应原性。用BrS_2菌株制备了3批Br—PPD,特异性蛋白含量均在95％以上,多糖、核酸含量均在3％以下。经Br—PPD与参考标准品标化后,其特异性和敏感性基本一致,两者反应曲线无显著差异。其它试验均达到Br—PPD生产规程要求。 A new purified Brucella-Brucella pure protein derivative, referred to as “Br-PPD”, was prepared from Brucella by using trichloroacetic acid (TCA) and ammonium sulfate (AS) Several animal experiments have shown that Br-PPD has good allergenicity. Three batches of Br-PPD were prepared by using BrS_2 strain. The specific protein contents were above 95%, and the contents of polysaccharides and nucleic acids were below 3%. After the standardization of Br-PPD and reference standard, the specificity and sensitivity are basically the same, no significant difference between the two reaction curves. Other tests have reached Br-PPD production rules and requirements.