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采用荧光染色技术对192例临床患有宫颈炎的病例进行宫颈涂片检查,检出宫颈上皮细胞内和中性粒细胞内衣原体包涵体32例(阳性率为16.6%),这些阳性标本分别进一步用姬姆萨和苏木精-伊红(HE)染色进行研究。结果表明,衣原体包涵体在姬姆萨染色的标本上得到证实;在HB染色的涂片上则不易发现;然而,在荧光方法染色的标本上,衣原体包涵体所发荧光的颜色明显不同于细胞结构发出的荧光颜色,在低倍荧光镜下就能识别.而且,该染色法对滴虫和癌细胞的识别亦具有一定的优越性。
Fluorescent staining was used to detect cervical smear in 192 cases of clinical cervicitis. 32 cases of inclusion bodies of cervical intraepithelial neutrophils and C.pneumoniae were detected (positive rate was 16.6%). These positive specimens The study was further performed with Giemsa and hematoxylin-eosin (HE) staining. The results showed that the inclusion bodies of Chlamydia were confirmed on Giemsa stained specimens but not on HB stained specimens. However, the fluorescent color of Chlamydia inclusion bodies on fluorescent-stained specimens was significantly different from that of cells The fluorescent color emitted by the structure can be identified under low magnification fluoroscopy. Moreover, the staining method for trichomoniasis and cancer cell identification also has some advantages.