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以番茄幼苗为试材,采用光合测试系统(CIRAS-2)和光纤光谱仪(QE65000),同步测定了不同占空比对0.04Hz和0.40 Hz频闪光照射下番茄叶片光合机构的动态影响。结果表明:与连续光相比,超低频(0.04Hz)不同占空比频闪光照射处理增加了番茄叶片RuBP底物的相对同化负荷(rAC),破坏了RuBP底物消耗与再生间的平衡和缓冲性能,降低了叶绿素荧光的光化学淬灭效率,抑制了光系统中电子传递活性,减少了脉冲驱动形成的同化力(NADPH和ATP),光合碳吸收呈现峰谷振荡的弛豫进程;此外,随着占空比的增加,番茄叶片实际光合光能转化效率(aLCE)呈现迅速上升至趋饱合的变化趋势,其中0.04Hz和0.40Hz频闪光下最大aLCE分别出现在60%和40%占空比下,但是当占空比小于60%,0.40Hz下各占空比的aLCE明显高于0.04Hz下的。
The effects of different duty cycles on the photosynthetic apparatus of tomato leaves at the frequency of 0.04 Hz and 0.40 Hz were measured synchronously with CIRAS-2 and QE65000 using tomato seedlings as test materials. The results showed that under low frequency (0.04Hz) and different duty cycles, stroboscopic irradiation increased the relative assimilation load (rAC) of RuBP substrates in tomato leaves, and ruined the balance between RuBP substrate consumption and regeneration and Buffer performance, decreased the photochemical quenching efficiency of chlorophyll fluorescence, inhibited the electron transfer activity in the light system, reduced the assimilation force (NADPH and ATP) formed by the pulse driving, and the photosynthetic carbon absorption presented the relaxation process of peak-valley oscillation; in addition, With the increase of the duty cycle, the actual photosynthetic light energy conversion efficiency (aLCE) of tomato leaves showed a trend of rapid increase to saturation. The maximum aLCE at the frequency of 0.04Hz and 0.40Hz strobes were 60% and 40% respectively However, when the duty cycle is less than 60%, the aLCE of each duty cycle at 0.40 Hz is significantly higher than that at 0.04 Hz.