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目的 :探讨及分析细胞因子[白介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)和γ-干扰素(IFN-γ)]干预后小鼠胰岛细胞株MIN6中的长链非编码RNA(lnc RNA)表达谱的变化,并进行初步验证。方法:细胞因子干预小鼠胰岛细胞株MIN6,流式细胞仪检测细胞凋亡,CCK-8检测细胞活性,采用Affymetrix Gene Chip Mouse Transcriptome Array 1.0基因芯片筛查细胞因子干预组和正常对照组差异表达的lnc RNA,并采用实时荧光定量PCR对筛选出的部分lnc RNA进行验证。结果:与对照组相比,细胞因子干预组的细胞凋亡增加,细胞活性下降。细胞因子干预组中表达相差2倍以上的lnc RNA有723条,其中表达上调有444条,表达下调有279条。表达相差5倍以上的lnc RNA有111条,其中表达上调有105条,表达下调有6条。表达相差1.5倍以上的m RNA有2 180条。10条lnc RNA在细胞因子干预组中的表达经初步证实,结果与芯片趋势一致。结论:细胞因子干预后小鼠胰岛细胞株MIN6中lnc RNA的表达谱发生了显著变化,这些差异表达的lnc RNA在细胞因子诱导的胰岛细胞凋亡中具有重要作用。
OBJECTIVE: To explore and analyze the effects of long-chain cytokines (IL-1β, TNF-α and IFN-γ) on mouse islet cell line MIN6 Non-coding RNA (lnc RNA) expression profile changes, and preliminary validation. Methods: Mouse pancreatic islet cell line MIN6 was treated with cytokines and the apoptosis was detected by flow cytometry. The cell viability was detected by CCK-8. Affymetrix Gene Chip Mouse Transcriptome Array 1.0 was used to screen the differential expression of cytokines in intervention group and normal control group Of lnc RNA, and the use of real-time fluorescence quantitative PCR screening of some lnc RNA validation. Results: Compared with the control group, the apoptosis of cytokine intervention group increased and the cell activity decreased. There were 723 lnc RNAs with a difference of more than 2 folds in the cytokine intervention group, of which 444 were up-regulated and 279 down-regulated. There were 111 lnc RNAs with more than 5-fold difference in expression, of which 105 were up-regulated and 6 down-regulated. There are 2 180 m RNAs that differ more than 1.5-fold in expression. Ten lnc RNA expression in cytokines intervention group was initially confirmed, the results and chip trends. CONCLUSION: The expression of lnc RNA in mouse islet cell line MIN6 significantly changes after cytokine intervention, and these differentially expressed lnc RNAs play an important role in cytokine-induced apoptosis of islet cells.