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目的 用 3种抗原检测国家戊型肝炎参比血清和急性戊型肝炎病人血清抗 -HEVIgG。方法 人工合成戊型肝炎病毒 (HEV)ORF2和ORF3多肽抗原 (BMU抗原 )检测参比血清的灵敏度 (90 0 % )、特异度 (10 0 0 % )和符合率 (97 5 % )均高于杆状病毒表达的HEV重组蛋白空粒子抗原 (JP抗原 ) (分别为 70 0 %、93 3%和 87 5 % )及大肠杆菌表达的重组蛋白抗原 (HKU抗原 ) (分别为 70 0 %、90 0 %和 85 0 % )。检测 84例急性戊型肝炎病人血清抗 -HEVIgG ,用BMU抗原检测抗-HEVIgG的阳性率为 10 0 % (84/ 84) ,高于JP抗原 (83/ 84,98 8% )和HKU抗原 (71/ 84,84 5 % )。结果 不同HEV抗原检测国家参比血清和急性戊型肝炎病人血清抗 -HEVIgG的灵敏度和特异性不同 ,其差异主要与编码该 3种抗原的HEV基因片段不同有关。ORF3抗原在检测急性HE病人中具有重要意义 ,联合应用ORF2和ORF3抗原可提高抗 -HEVIgG检出率。 结论 抗 -HEVIgGELISA诊断试剂至少应包括ORF2和ORF3 2种抗原
Objective To detect serum anti-HEVIgG in national hepatitis E reference serum and acute hepatitis E patients by using three antigens. Methods The sensitivity (90%), specificity (100%) and coincidence rate (97.5%) of artificial HEV ORF3 polypeptide antigen (BMU antigen) Baculovirus-expressed HEV recombinant protein empty particle antigen (JP antigen) (70 0%, 93 3% and 87 5%, respectively) and E. coli expressed recombinant protein antigen (HKU antigen 70 0%, 90 0% and 85 0%). The serum anti-HEVIgG levels in 84 patients with acute hepatitis E were detected. The positive rate of anti-HEVIgG detected by BMU antigen was 100% (84/84), higher than that of JP antigen (83/84, 988%) and HKU antigen 71/84, 84 5%). Results The sensitivity and specificity of different HEV antigens for detecting serum anti-HEVIgG in national reference serum and acute hepatitis E patients were mainly different from those of HEV gene segments encoding the three antigens. ORF3 antigen in the detection of acute HE patients is of great significance, combination of ORF2 and ORF3 antigen can increase the detection rate of anti-HEVIgG. Conclusion Anti-HEVIgGELISA diagnostic reagents should include at least ORF2 and ORF3 2 kinds of antigens