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目的通过观察人参二醇组皂苷(PDS)对TRPV6蛋白的影响,探讨PDS对LPS诱导休克时脑损伤的保护机制。方法将大鼠随机分为对照组、LPS休克(LPS)组、地塞米松(LPS+Dex)组、PDS低剂量(LPS+PDSL)组、PDS中剂量(LPS+PDSM)组及PDS大剂量(LPS+PDSH)组。大鼠静脉注射LPS(4 mg/kg)4 h后,观察各组大鼠生存期,计算生存率,Western Blot检测大脑皮质中TRPV6和Bcl-2的蛋白表达。结果与对照组相比,LPS组大鼠生存率降低达50%,脑皮质中TRPV6的蛋白表达水平明显降低,Bcl-2/Bax蛋白表达的比值降低;而与LPS组相比,LPS+Dex组和LPS+PDS各组大鼠生存率增加,脑皮质中TRPV6的蛋白表达水平明显增高,Bcl-2/Bax比值也增高。结论PDS减轻LPS诱导的脑损伤,可能与上调脑皮质中TRPV6蛋白有关。
Objective To investigate the protective effect of PDS on brain injury induced by LPS during shock by observing the effect of panaxadiol saponins (PDS) on TRPV6 protein. Methods Rats were randomly divided into control group, LPS shock (LPS) group, dexamethasone (LPS+Dex) group, PDS low dose (LPS+PDSL) group, PDS middle dose (LPS+PDSM) group and PDS high dose (LPS+PDSH) group. LPS (4 mg/kg) was injected intravenously in rats for 4 h. The survival time of each group was observed and the survival rate was calculated. Western Blot was used to detect the expression of TRPV6 and Bcl-2 proteins in cerebral cortex. Results Compared with the control group, the survival rate of LPS group was reduced by 50%, the protein expression level of TRPV6 in cerebral cortex was significantly decreased, and the ratio of Bcl-2/Bax protein expression was decreased. Compared with LPS group, LPS+Dex The survival rate of rats in each group and LPS+PDS group increased, the protein expression level of TRPV6 in the cerebral cortex was significantly increased, and the Bcl-2/Bax ratio was also increased. Conclusion PDS can reduce LPS-induced brain injury and may be related to the up-regulation of TRPV6 protein in cerebral cortex.