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甘蓝型油菜自交不亲和临保材料在杂种优势利用和芸薹属自交不亲和性的机理研究方面具有重要的价值。以自交不亲和系SI1300为受体、临保系97-wen238为供体,通过不亲和植株与供体连续回交构建BC6、BC7和BC8群体对临保性的遗传进行了初步的分析,并检出与其连锁的分子标记。SI1300×97-wen238 F1的平均亲和指数小于1,表明97-wen238为甘蓝型油菜自交不亲和临保系。BC6、BC7和BC8群体的亲和指数集中分布在小于2和大于10两端,以亲和指数小于2为不亲和、大于10为亲和,3个回交世代不亲和植株与亲和植株的分离比均符合1∶1,说明97-wen238的临保性受1对基因控制。以包含140个体的BC7为作图群体,利用集团选择法,通过筛选1 280对AFLP引物组合,得到了与临保性两侧连锁的3个AFLP标记MC07/PO02、MC14/PO04和EA12/MG07,遗传距离分别为7.8 cM、11.7 cM和6.6 cM,为标记辅助选择自交不亲和临保系奠定了基础。
Brassica napus L. self-incompatibility and environmental protection material has the important value in the study of heterosis utilization and the mechanism of self-incompatibility of Brassica. The self-incompatible line SI1300 was used as donor and Pro-line 97-wen238 was used as donor. The progeny inheritance of BC6, BC7 and BC8 was constructed by continuous backcrossing of incompatible plants and donors Analysis, and detect linked to its molecular markers. The mean affinity index of SI1300 × 97-wen238 F1 was less than 1, indicating that 97-wen238 was self-incompatible Progeny of Brassica napus. The affinity index of BC6, BC7 and BC8 populations concentrated on the two ends of less than 2 and more than 10, the affinity index less than 2 was not affinity, more than 10 was affinity, 3 backcross generation of incompatible plants and affinity Plant isolation ratio are in line with 1: 1, indicating 97-wen238 of the genetically controlled by a pair of genes. Using BC7 containing 140 individuals as the mapping population, three AFLP markers MC07 / PO02, MC14 / PO04 and EA12 / MG07 linked to both sides of the PIC were obtained by using the group selection method by screening 1280 AFLP primer combinations. , Respectively. The genetic distances were 7.8 cM, 11.7 cM and 6.6 cM, respectively, which laid the foundation for the marker-assisted selection of self-incompatible and in-line protection.